Screening Framework Guidance for Providers of Synthetic Double-Stranded DNA: This guidance sets forth recommended baseline standards for the gene and genome synthesis industry (providers) and for manufacturers of benchtop nucleic acid synthesis devices. It also details best practices for customers of synthetic nucleic acids (i.e., institutions, principal users, end users, and third-party vendors) regarding screening orders for sequences of concern and for responsibly handling the use and transfer of synthetic nucleic acids containing sequences of concern. In addition, this guidance seeks to encourage best practices to address biosecurity concerns associated with the potential misuse of synthetic nucleic acids to bypass existing regulatory controls.